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Culture Myograph System Model 202CM

25x Concentrated PSS (1000ml)

Solution 1

Chemical MW (g/mol) Conc. (mmol/l) Conc. (g/l)

NaCl 58.44 118.99 173.850

KCl 74.56 4.69 8.750

CaCl

- 2H

O 147.02 2.50 9.200

Solution 2

Chemical MW (g/mol) Conc. (mmol/l) Conc. (g/l)

MgSO

- 7H

O 246.48 1.17 7.225

KH2PO

136.09 1.18 4.025

Solution 3

Chemical MW (g/mol) Conc. (mmol/l) Conc. (g/l)

EDTA 372.24 0.03 0.250

a) Dissolve the chemicals for solution 1 in about 800ml double distilled H

O in a

1000ml graduated bottle. Dissolve the chemicals for solutions 2 and 3 in 75ml

double distilled H

O in individually cylinders. Gently heat solution 3 to dissolve

the EDTA.

b) Solution 2 and 3 is added to solution 1 and the graduated bottle is lled with

additional double distilled H

O to reach a nal volume of 1000.0ml.

Before use:

c) Dilute the 25 x PSS stock solution 1:25 using double distilled H

d) Add

1.091 g/l Glucose

2.100 g/l NaHCO

e) Aerate the solution with carbogen (95%O

+ 5%CO

) for at least 20 minutes. If

necessary wait further for the pH of the buffer to reach pH 7.4.

1 2 ... 51 52 53 54 55 56 57 58 59 60 61 ... 65 66

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DMT Presenter Control Manual de usuario Pagina 56