
Note:
A sterile micro lter (0.20µm) is connected between the pressure manometer
reservoir and the two perfusion reservoirs to prevent contamination of the perfusion
buffer.
2.2.2 Perfusion Flow Control
Regulating the difference in height between the two ow reservoirs allows control of
the perfusion ow velocity. The principle is illustrated in Fig. 2.3. In Fig. 2.3A the two
reservoirs are equal in height and no ow will occur. In Fig. 2.3B the difference in
height reveals a ow from the right reservoir to the left reservoir.
Note:
To enable the perfusion ow, it is important that the right glass cannula is connected
to the long steel pipe in the right reservoir (marked by the arrow in Fig. 2.3A.).
Figure 2.3A and B Illustration of perfusion ow control
21
User Manual vers. 1.4 Chapter 2
Comentarios a estos manuales