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Culture Myograph System Model 202CM

7.2 Mounting Protocol for Small Arteries

This section shortly describes the basic technique of mounting small vessels in the

culture myograph:

• One end of the vessel is care-fully mounted and secured (with two ne nylon

sutures) to one of the hollow glass micro-cannulas.

• The lumen is ushed gently with PSS to remove any blood or debris.

• The second end of the vessel is then mounted and secured to the second

micro-cannula.

• The PSS in the myograph chamber is gradually warmed to 37

C over a periode

of 15 minutes.

Figure 7.6 Mounting of vessel in the

culture myograph

Figure 7.7 Vessel secured to the two glass

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DMT Presenter Control Manual de usuario Pagina 54